Zagga, Abdullahi Daudu Usmanu Danfodiyo University - College of Health Sciences - Department of Anatomy, Nigeria , Ismail, Somaia Muhammad National Research Centre - Department of Medical Molecular Genetics, Division of Human Genetics and Genome Research, Egypt , Tadros, Aziz Abdo Usmanu Danfodiyo University - College of Health Sciences - Department of Anatomy, Nigeria , Oon, Hamidu Ahmed Usmanu Danfodiyo University - College of Health Sciences - Department of Paediatrics, Nigeria

An Efficient Method of Extracting PCR Amplifiable DNA from Human Skeletal Fragments and Teeth Specimens

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Purpose:Extraction and successful PCR amplification of DNA from humanremains in historical and forensic cases have great importance. The purpose of this study is to demonstrate the efficiency of a method of DNA extraction and PCR amplification of embalmed dried human cadaveric skeletal fragments and teeth specimens from Sokoto, Northwestern Nigeria.Materials and Methods: The efficiency of a method of DNA extraction and PCR amplification was tested on thirteen (13) embalmed dried human cadaveric skeletal fragments and nine (9) teeth specimens from Sokoto, Northwestern Nigeria were used in the study.Results: Of the 13 embalmed dried human cadaveric skeletal fragments, 12 (92.3%) samples amplified with apparent bands. For the 9 embalmed dried human teeth specimens, 7 (78%) samples amplified with apparent bands.Conclusion: The study has shown that our method of DNA extraction and PCR amplification was efficient on embalmed dried human cadaveric skeletal fragments and teeth specimens, from Sokoto, Northwestern Nigeria.

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Extraction , PCR amplifiable DNA , embalmed , dried human cadaveric bones and teeth

Cukurova Medical Journal

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