مرکز منطقه ای اطلاع رساني علوم و فناوري - INVESTIGATION OF MMP-2 AND MMP-13 IMMUNOREACTIVITY IN PLACENTA OF RATS TREATED WITH RETINOIC ACID

Abstract :

Objective: Matrix metalloproteinases (MMPs) are enzymes and play important roles at implantation by degradation of extracellular matrix. Retinoic acid (RA) is an important molecule has effects on MMP synthesis, embriyonic development and trophoblast invasion at placentation. In this study we investigated the MMP-2 and MMP-13 immunoreactivity on rat placenta treated by RA. Materials and Methods: In this study 16 female Spraque Dawley rats were used, divided equally for control (8) and RA (8) treated groups. Rats were treated by 13-cis RA (40mg/kg/day) by gavaj for 3 days. For immunochemical study we used rabbit polyclonal MMP-13 and mouse monoclonal MMP-2 antibody. Results: In the control group the trophoblast giant cells show ed strong (+++) and chorioamniotic membrane moderate (++) positivity for MMP-2. There was no MMP-2 immunoreactivity detected at trophoblastic giant cells of RA treated group in contrast to the strong positivity were detected at chorion epithelium. Moderate MMP-13 immunoreactivity was detected at trophoblastic giant cells and chorioamniotic membrane of control group. In RA treated group trophoblastic giant cells showed moderate, chorioamniotic membrane showed strong reactivity for MMP-13. Conclusion: It is convinced that exogenously applied retinoic acid affects implantation negatively by repressing MMP-2 synthesis in trophoblastic giant cells and decidua, impair membrane stabilization by increasing MMP-2 and MMP-13 synthesis in chorioamniotic membrane and therefore prevent survival.