Author/Authors :
SENKAL, İbrahim Volkan Yeditepe Üniversitesi - Tıp Fakültesi - İç Hastalıkları Anabilim Dalı, Turkey , AKYÜZ, Ümit Yeditepe Üniversitesi - Tıp Fakültesi - iç Hastalıkları Anabilim Dalı, Turkey , YALNIZ, Fırat Yeditepe Üniversitesi - Tıp Fakültesi - iç Hastalıkları Anabilim Dalı, Turkey , ÇiFTÇi, Ferda Yeditepe Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, Turkey , YURTSEVER, Aysel istanbul Teknik Üniversitesi - ONKIM Laboratuarları - Kök hücre Teknolojileri Bölümü, Turkey , PATA, Cengiz
Title Of Article :
EFFECTS OF MESENCHYMAL STEM CELLS ON TISSUE INJURY, INFLAMMATORY AND ANTI-INFLAMMATORY CYTOKINES IN CROHN’S DISEASE MODEL IN RATS
Abstract :
Background: Mesenchymal stem cells (MSC) have immune supressive effects by decreasing inflammatory cytokines (IFN-γ, TNF-α) increasing antiinflammatory cytokines (IL-10) and inhibiting T cells and antigen presenting cells. Various cytokines are responsible for mucosal damage in Crohn‘s disease and TNF-α is one of the most important of them. The aim of this study is to determine the role of MSC in the treatment of Crohn‘s disease model.Methods: 3% Dextran sulphate sodium (DSS) is used to make Crohn‘s disease model. There are 7 groups which composed of 6 rats in each group; Group 1: SHAM group, Group 2: DSS+ placebo, Group 3: DSS+ methylprednisolone 1 mg/kg, po, Group 4: DSS + mesalazine 100 mg/kg, po., Group 5: DSS+ MKH, 106 cell/rat, intraperitoneal, Group 6: DSS + methylprednisolone 1 mg/kg, po + MSC, 10^6 cell/rat, intraperitoneal, Group 7: DSS + mesalazine 100 mg/kg, po + MSC, 10%6 cell/rat, intraperitoneal. Groups are compared with the alteration of pathological and biochemical (TNF, INF IL-10) parameters. Results: The average scores of group 1, 2 and 3 were higher than the average scores of Group 5, 6 and 7 significantly in pathological evaluation (p 0.05). IL-10 was 108.33±2.23 pg/ml in Group 1, 80.83±3.62 pg/ml in Group 2 where the levels of IL-10 were 131.66±3.83 pg/ml, 138.33±3.19 pg/ml and 115.33±5.21 pg/ml in Group 5, 6 and 7 respectively, in which MSC was used as a treatment. It is observed that the average of these three groups were significantly higher (p 0.05). Conclusion: This study suggest that MSC may take a role in the treatment of Crohn‘s disease by increasing the amount of IL-10.
NaturalLanguageKeyword :
Mesenchymal stem cells , inflammatory bowel disease
JournalTitle :
Journal Of Istanbul Faculty Of Medicine
