Kara, Nihal Beldüz Ordu Üniversitesi - Diş Hekimliği Fakültesi - Pedodonti Anabilim Dalı, Turkey , Reis, Ahu Kamburoğlu Karadeniz Teknik Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji Anabilim Dali, Turkey , Yılmaz, Yücel Atatürk University - Dis Hekimligi Fakültesi - Pedodonti Anabilim Dali, Turkey , Tosun, İlknur Karadeniz Teknik Üniversitesi - Tip Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey

In vitro study of Candida albicans adhesion and biofilm formation on various dental restorative material surfaces: a pilot study

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OBJECTIVE: Candida adhesion to any oral substrata is the first and the essential stage in the formation of a fungal biofilm. This study aimed to compare the adherence of Candida albicans cells to 11 restorative materials. MATERIALS AND METHOD: Disc-shaped specimens of each material were prepared according to the manufacturers’ instructions. Amalgam specimens were used as control. The specimens were placed in contact with C. albicans cultures on agar plates and inhibition zones around the specimens were evaluated. In order to evaluate biofilm formation, XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyla mino) carbonyl]-2H-tetrazolium hydroxide} technique and scanning electron microscopy (SEM) were used. RESULTS: No inhibition zone was observed around the specimens. According to XTT assays, the glass ionomer cement (Ionofil molar) revealed the least biofilm formation whereas the amalgam (ANA 2000) revealed the highest XTT value. In the SEM examination, the amount of C. albicans growth was shown significantly less on the resin modified glass ionomer (Ionolux AC) and glass ionomer cement (Fuji IX GP) specimens. The two compomers (Twinky Star, Dyract Extra) and the composite (Grandio SO) specimens exhibited greater number of C. albicans cells adhering to the surfaces. CONCLUSION: The results of the study point to use of glass ionomer cements if reduced C. albicans adhesion to dental restorative materials is aimed; this may be particularly important in patients with neutropenia, cancer or compromised immune system.

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Adhesion , biofilm , Candida albicans , fungus

Acta Odontologica Turcica

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