Author/Authors :
ESEN, BURAK Istanbul Üniversitesi - DETAE MOLEKÜLER TIP ANABILIM DALI, Turkey , DASDEMIR, SELÇUK İstanbul Üniversitesi - DETAE MOLEKÜLER TIP ANABILIM DALI, Turkey , ÇINÇIN, ZEYNEP BIRSU İstanbul Üniversitesi - DETAE MOLEKÜLER TIP ANABILIM DALI, Turkey , ÖZDEMIRCAN, ABDULLAH İstanbul Üniversitesi - DETAE MOLEKÜLER TIP ANABILIM DALI, Turkey , ÇAKMAKOGLU, BEDIA İstanbul Üniversitesi - DETAE MOLEKÜLER TIP ANABILIM DALI, Turkey
Abstract :
Osteoprotegerin (OPG) is a recently identified cytokine that acts as a receptor for the receptor activator of the NF-KB ligand (RANKL). OPG T950C polymorphism has been reported to be associated on diabetes mellitus risk. We used PCR, RFLP and gel electrophoresis techniques to detect OPG T950C polymorphism on 77 type II diabetic patients and 83 healthy controls. Frequencies of OPG T950C genotypes were 41,56% TT, 15,58% CC, 42,86% TC in patient group and 31,62% TT, 14,70% CC, 53,68% TC in control group. We have not detected any remarkable statistical differences between control and patient group in OPG T950C genotypes and alleles frequencies (p 0.05). In addition, OPG T950C CC genotype frequencies increase 3,15 times at existence of myokard infractus (MI), 1,38 times at existence of left ventricular hypertrophy (LVH), 4 times in individuals who had familiy history of DM, 2,6 times in men, 2,33 times in hypertansives, 2,3 times in individuals who had familiy history of coroner arter disease (CAD). The increase of CC genotypes frequencies at existence of factors related to development of CAD thought us that this genotype could be a risk factor. In conclusion, it was detected that OPG T950C polymorphism could be related to CAD in diabetic patients in our study.
