Author/Authors :
Ktigtiksezer, Umut Can istanbul Universitesi - Deneysel Tip Aragtirma Enstitiisu - Immunoloji AD, Turkiye , Tahrali, Ilhan istanbul Universitesi - Deneysel Tip Aragtirma Enstitiisu - Immunoloji AD, Turkiye , Bilgig-Gazioglu, Sema istanbul Universitesi - Deneysel Tip Aragtirma Enstitiisu - Immunoloji AD, Turkiye , Cetin, Esin Aktas istanbul Universitesi - Deneysel Tip Aragtirma Enstitiisu - Immunoloji AD, Turkiye , Gull, Ahmet Istanbul Universitesi - Istanbul Tip Fakiiltesi - Romatoloji BD, Ig Hastaliklan AD, Turkiye , Tugal-Tutkun, Ilknur Istanbul Universitesi - Istanbul Tip Fakiiltesi - Goz Hastaliklan AD, Turkiye , Deniz, Gtinnur istanbul Universitesi - Deneysel Tip Aragtirma Enstitiisu - Immunoloji AD, Turkiye
Abstract :
Natural Killer (NK) cells are members of innate immunity who defend against viral infections and tumors with their cytotoxic activity and also regulate immune responses with their cytokioe secretions. Different sub-groups of NK cells defined are shown to have initiating or limiting effects on pathogenesis of various diseases. According to research requirements, it is essential to investigate pure NK cells in some experimental setups. This study investigated purity rates of NK cells, isolated by a magnetic activated cell sorting method, in relapse and remission phases of follow-up Behçet s disease patients, as well as healthy controls. Our findings show that NK cells were isolated with a high purity rate both at patients and healthy controls, with no significant difference. In repeated purification of follow-up patients in relapse and remission periods, there were no differences of purity in between two periods, but there were differences between each individual patient. This difference between each individual may arise from different binding specificities of monoclonal antibodies, which vary between each individual. New developments in this area may diminish variabilities and provide increased purity rates.
NaturalLanguageKeyword :
Behçet s disease , uveitis , NK cells , attack , remission
