Karagedik, E. Hande Istanbul Üniversitesi - Deneysel Tıp Araştırma Enstitüsü - Moleküler Tıp Anabilim Dalı, Turkey , Atalan, Nazan Dr. Siyami Ersek Göğüs Kalp Ve Damar Cerrahisi Eğitim Ve Araştırma Hastanesi - Anestezi ve Reanimasyon Klinigi, Turkey , Acari, Leyla Istanbul Üniversitesi - Deneysel Tıp Araştırma Enstitüsü - Moleküler Tıp Anabilim Dalı, Turkey , Ergent, H. Arzu Istanbul Üniversitesi - Deneysel Tıp Araştırma Enstitüsü - Moleküler Tıp Anabilim Dalı, Turkey

ANALYSIS OF TOLL-LIKE RECEPTOR-2 IN SEPSIS

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Sepsis is identified as the systemic inflammatory response developed against infections. Studies showed that humoral immune system is activated and various cytokines are released due to infections in tissues and traumatic damage. Immune system is a defensive system divided as innate and adaptive. Toll-like receptors (TLR) are transmembrane proteins, which form the innate immune response against many pathogens and play an important role on host immunity by together activating the adaptive immune response. A member of this family, the TLR-2 peptidoglycan may respond by binding with lipoticoic acid, gram-positive LPS and various gram-positive macromolecules. In this study it was aimed to investigate the possible effect of TLR-2 polymorphisms on serum levels with clinic and prognostic parameters of sepsis by determining the TRL-2 Arg753Gln and del -196-174 gene polymorphisms and TLR-2 serum levels. Thereby, 59 sepsis patients and 83 healthy controls were included to this study. In order to determine TLR- 2 polymorphisms, the PCR-RFLP method was performed and serum TLR-2 levels were measured by ELISA technique. No statistically significant differences were found in these two polymorphisms between patient and control groups (p 0,05). On the other hand a statistically significant increase of serum TLR levels were detected in patients compared to healthy controls (p 0,001). Finally, this is the first study on these two polymorphisms conducted on sepsis patients in our country. Our results showed no association between sepsis risk and these polymorphisms. On the other hand, the serum TLR levels were elevated in the patient group independent from the del -196- 174 polymorphism existence and the GG and G alleles of the Aig753Gln polymorphism may be used as a predictor of elevated TLR-2 levels in sepsis patients.

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