Altas, Mustafa Celal Bayar University - Medical Faculty - Department of Biochemistry, Turkey , Var, Ahmet Celal Bayar University - Medical Faculty - Department of Biochemistry, Turkey , Köse, Can Celal Bayar University - Medical Faculty - Department of Histology, Turkey , Özbilgin, Kemal Celal Bayar University - Medical Faculty - Department of Histology, Turkey , Arı, Zeki Celal Bayar University - Medical Faculty - Department of Biochemistry, Turkey

Endothelial dysfunction in high fructose containing diet fed rats: Increased nitric oxide and decreased endothelin-1 levels in liver tissue

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Objectives: Dietary high fructose consumption which is closely associated with endothelial dysfunction via insulin resistance has recently increased in developed countries. Insulin resistance has a promoter effect on many metabolic disorders such as syndrome X, polycystic ovary syndrome, Type 2 diabetes mellitus etc. Our aim in this study is to understand the impact of increased fructose intake on metabolisms of glucose, insulin and endothelial dysfunction by measuring nitric oxide (NO) and endothelin-1 (ET-1) levels in hepatic tissue which is crucial in fructose metabolism. Materials and Methods: We designed an animal study to understand increased fructose intake on hepatic endothelium. Twenty adult male albino rats were divided into two groups; the study group (group 1, n=10) received isocaloric fructose enriched diet (fructose-fed rats, containing 18.3% protein, 60.3% fructose and 5.2% fat) while the control group received purifi ed regular chow (group 2, n=10) for 2 weeks. After feeding period, blood and hepatic tissue samples were collected and glucose, insulin, NO and ET-1 levels were analysed. Results: We found increased fasting glucose and insulin levels and impaired glucose tolerance in fructose fed rats. Higher NO and lower ET–1 levels were also detected in hepatic tissue samples of the group 1. Conclusion: Increased fructose consumption has deleterious effects on glucose tolerance, insulin resistance and may cause to endothelial dysfunction.

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Fructose consumption , endothelial dysfunction , nitric oxide , endothelin , 1 , liver

Dicle Medical Journal

198