Kökçam, İbrahim Fırat Üniversitesi - Tıp Fakültesi - Deri ve Zührevi Hastalıkları Anabilim Dalı, Turkey , Dilek, Nursel Fırat Üniversitesi - Tıp Fakültesi - Deri ve Zührevi Hastalıkları Anabilim Dalı, Turkey , Gödekmerdan, Ahmet Fırat Üniversitesi - Tıp Fakültesi - İmmünoloji Anabilim Dalı, Turkey , Akpolat, Nusret Fırat Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, Turkey , İlhan, Fulya Fırat Üniversitesi - Tıp Fakültesi - İmmünoloji Anabilim Dalı, Turkey

T lymphocytes in the lesional skin and the levels of peripheral blood cytokines in patients with psoriasis

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Objective: In the current study, it was aimed to investigate the roles of tissue cellular immunity and serum levels of cytokines in the patients with plaque psoriasis treated with calcipotriol-betamethasone dipropionate.Materials and methods: The study included 20 patients with psoriasis. Peripheral blood and biopsy samples were collected from lesional and normal skins before and after treatment. The results were compared with each other.Results: Immunohistochemical examination revealed significant elevations of CD4+, CD8+ and CD25+ T lymphocytes in the lesional tissues when compared to that in the healthy tissues and post treatment tissue (p 0.001, p=0.013 and p 0.001, respectively). These lymphocytes in the lesional tissues at post-treatment period were not higher than those in the healthy tissues (p 0.05). The levels of IL–4, IL–10, TNF-α, IFN-γ and TGF-β1 in serum were not significantly different between before and after treatment periods (p 0.05).Conclusion: Our study demonstrated that there were infiltration of CD4+ and CD8+ cell in the lesional skin and CD8+ T-lymphocytes were the dominant cell types. The improvement of the lesions and significant decreases in CD4+ and CD8+ T-cells in accordance with the treatment strongly support the hypothesis that Th lymphocytes may have prominent roles in the immunopathogenesis of the disease. However, our findings showed that sufficient T-cells still remains in the tissue, which is consistent with the chronic characteristic of the disease, and the topical treatment could not be able to prevent the activation of the disease.

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Psoriasis , pathogenesis , cell , mediated immunity , T , lymphocytes , cytokines

Dicle Medical Journal

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