Author/Authors :
İnci, Melek Tıbbi Mikrobiyoloji A.D. - Tıp Fakültesi - Mustafa Kemal Üniversitesi, Turkey , Atalay, Mustafa Altay Erciyes Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Türkiye , Koç, Ayşe Nedret Erciyes Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Türkiye , Özer, Burçin Mustafa Kemal Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji A D, Turkey , Kılınç, Çetin Mustafa Kemal Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Türkiye , Durmaz, Süleyman Erciyes Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Türkiye
Abstract :
Objectives: In the present study, it was aimed to compare results obtained by using VITEK 2 YST Card (bioMérieux, France) with those obtained by using API 20C AUX (bioMérieux, France) for identification of non- albicans Candida species, which was isolated from various clinical samples, at level of species. Materials and methods: Forty-one non-albicans Candida isolates, which were isolated from 28 urine, 10 blood and 3 vaginal swab specimens, and found to be negative by germ tube test, were identified by using VITEK 2 YST Card (bioMérieux, France). In addition, microscopic morphology was assessed in corn-meal Tween 80 agar, while carbohydrate assimilation was assessed by using commercially available API 20C AUX kit (bioMérieux, France). Results: Thirty-four isolates (82.9%) were identified as identical species by these 2 systems, while different results were obtained in 7 isolates (17.1%). 5 isolates, identified as Candida glabrata by API 20C AUX system, were identified as Candida tropicalis (n=2), Candida krusei, Candida lipolitica and Candida kefyr by VITEK 2 YST Card. One other isolate, identified as C.tropicalis, was identified as Candida parapsilosis; and additional one isolate, identified as C.parapsilosis, was identified as C.tropicalis. Conclusion: It was concluded that one should be cautious in the identification of C.glabrata, in particular, C.tropicalis and C.parapsilosis, although between VITEK 2 YST Card and API 20C AUX system results was found largely similarity in identification of non-albicans Candida spp.
NaturalLanguageKeyword :
Non , albicans Candida spp. , identification , phenotypic systems.
