Ülker, Hayriye Esra Selçuk Üniversitesi - Dis Hekimligi Fakültesi - Restoratif Dis Tedavisi Anabilim Dali, Turkey , Ülker, Mustafa Selçuk Üniversitesi - Dis Hekimligi Fakültesi - Restoratif Dis Tedavisi Anabilim Dali, Turkey , Yalçın, Muhammet Inönü Üniversitesi - Dis Hekimligi Fakültesi - Restoratif Dis Tedavisi Anabilim Dali, Turkey , Dündar, Ayşe Abant Izzet Baysal Üniversitesi - Dis Hekimligi Fakültesi - Restoratif Dis Tedavisi Anabilim Dali, Turkey

Cytotoxicity of fissure sealants in vitro

26686

OBJECTIVE: This in vitro study aimed to evaluate the cytotoxicity of three different fissure sealant materials. MATERIALS AND METHOD: Cylindrical-shaped (2x5 mm) fissure sealant test samples were prepared according to the manufacturers directions (Fuji Triage, Dyract Seal, UltraSeal XT Plus). L929 mouse fibroblast cells were plated in 96- well plates, and maintained in an incubator at a mixture of 5% CO2/95% air and at 37 °C for 24 h. The test samples were immersed in Basal Medium Eagle (BME) with 10% New Born Calf Serum and 5% penicillin/streptomycin. After 24 h, the incubation medium of the cells was replaced by the medium in which the test samples were extracted. Cells that were not exposed to the material extracts were used as control. After 24 h, cell viability was determined by using MTT assay. The data were analyzed by One-Way ANOVA and Tukey s HSD Post-hoc tests. RESULTS: Dyract Seal and Fuji Triage were significantly different from the control (p 0.05). UltraSeal XT Plus was similar to the control (p 0.05). Dyract Seal presented the greatest cytotoxic effect on the L929 cells. CONCLUSION: The results of this study indicate that fissure sealant materials can release toxic agents. Biocompatibility, like for other restorative materials, is an important aspect for fissure sealants.

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Compomer , composite resin , cytotoxicity , fissure sealant , glass ionomer

Acta Odontologica Turcica

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