Çakırca, Gökhan Ceylanpınar State Hospital - Department of Biochemistry, Turkey , Evliyaoğlu, Osman Dicle Üniversitesi - Tıp Fakültesi - Tıbbi Biyokimya AD, Türkiye , Polat, Cemal Dicle Üniversitesi - Tıp Fakültesi - Tıbbi Biyokimya AD, Türkiye , Işık, Fatma Birgül Dicle Üniversitesi - Tıp Fakültesi - Tıbbi Biyokimya AD, Türkiye , Mete, Nuriye Dicle University - Faculty of Medicine - Department of Biochemistry, Turkey

Standardization and performance evaluation of manual measurement method for paraoxonase activity

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Objectives: We hypothesised that using the traditional laboratory methods for preparing the kit with chemicals may represent sufficient analytic performance with commercial kits, because of the fresh reagents and cost effective. We aimed to apply the paraoxonase (PON1) study procedure to the autoanalyser and evaluate the analytic performance of the study. Methods: Thirty five healthy individuals were included in the study. A total of 100 mL serum pool was created after centrifugation of venous blood samples. To assess the intra-assay variations of PON1 enzyme activities 20 samples and for inter-assay variations 30 samples were prepared from the serum pool and PON1 enzyme activities were studied. As well as, 30 samples were studied to evaluate the correlation between the commercial kit and the manual prepared kit from chemicals. Results: In this study, the mean, standard deviation (SD), percent coefficient of variation (% CV) values of PON1 enzyme activities for intra-assay (72.70 U / L, 0.43, 0.59) and inter-assay (74.24 U / L, 3.00, 4.04) were determined. The correlation coefficient between the manual method and the commercial kit were r = 0.92 and r2 = 0.84. Conclusion: The results of manual kit were showed good analytical performance with commercial kit. Therefore, the serum pool which was partially standardized in this study was portionized for using as an internal quality control serum for future studies in our laboratory. However, the use of standardized control sera, is considered to be more reliable in routine laboratories.

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Paraoxonase , standardization , internal quality control

Dicle Medical Journal

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