Saraymen, Berkay Erciyes University - Faculty of Medicine - Department of Immunology, Department of Biochemistry, Turkey , Çimen, Behzat Erciyes University - Faculty of Pharmacy - Department of Biochemistry, Turkey , Çetin, İhsan Batman University - School of Health - Department of Nutrition and Dietetics, Turkey , Köker, Mustafa Yavuz Erciyes University - Faculty of Medicine - Department of Immunology, Turkey , Çetin, Aysun Erciyes University - Faculty of Medicine - Department of Biochemistry, Turkey , Eser, Bülent Erciyes University - Hematology-Oncology Hospital - Department of Hematology, Turkey

Determination of P-Glycoprotein Expression by Flow Cytometry in Hematological Malignancies

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Objective: Determination the expression of P-glycoprotein is especially problematic for normal tissues because immunological methods are limited in terms of sensitivity. We aimed to determine the expression of P-glycoprotein and CD34 by flow cytometry, and to evaluate the level of expression of Pglycoprotein and CD34 with unresponsive to treatment in patients diagnosed with hematologic malignancy. Methods: Our study included fifty patients diagnosed with acute myeloblastic leukemia and acute lymphoblastic leukemia, and twenty healthy controls who were admitted to Erciyes University Hematology-Oncology Hospital. The suspended cells from bone marrow samples of patients and the peripheral blood samples of healthy people were marked with P-glycoprotein phycoerythrin and CD34 FITC or PerCP Cy 5.5; and then surface expression was measured by means of flow cytometry. Results: In 6 of 30 acute myeloblastic leukemia patients Pglycoprotein and CD34 expression, in 6 of 20 acute lymphoblastic leukemia patients P-glycoprotein, in 5 of them CD34 expression were determined. A significant relation between P-glycoprotein and CD34 expressions in acute myeloblastic leukemia and acute lymphoblastic leukemia bone marrow samples was reported. Conclusion: Our data indicate that flow cytometry is more reliable, precise and faster than molecular methods for measuring P-glycoprotein expression and suggests the possibility of a significant relationship between P-glycoprotein and CD34 expressions in acute myeloblastic leukemia and acute lymphoblastic leukemia bone marrow samples. The blast cells expressing CD34 on their surface along with Pglycoprotein simultaneously show that multi drug resistance 1 gene is mostly active in immature cells.

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MDR , 1 gene , AML , ALL , P , glycoprotein , flow cytometry

Dicle Medical Journal

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