CEYLAN, Ahmet Erciyes Üniversitesi - Fen Fakültesi - Biyoloji Bölümü, Turkey , GÜRCAN, Kahraman Erciyes Üniversitesi - Ziraat Fakültesi - Tarımsal Biyoteknoloji Bölümü, Bitkisel Biyoteknoloji Ana Bilim Dalı, Turkey , AKBULUT, Mikail Erciyes Üniversitesi - Fen Fakültesi - Biyoloji Bölümü, Turkey , GHADERİ, Maryam Erciyes Üniversitesi - Fen Bilimleri enstitüsü, Turkey

Prevalence of Sharka (Plum Pox Virus) in Kayseri

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Sharka cuased by Plum Pox virus is the most detrimental disease of stone fruits. Existence of PPV in Kayseri was reported previously. However prevalence of PPV was unknown to the date. Surveys were conducted and symptoms on stone fruits were observed to determine and assess the prevalence of Sharka disease in Kayseri. Fifty three samples showing the symptoms in Kayseri city center were collected randomly. In Yahyalı, 10 samples including symptomless samples in an infected plum orchard were collected. Double Antibody Sandwich Indirect-ELISA (DASI-ELISA) and Reverse Transcriptase (RT) PCR were performed to test the virus existence. PPV-D specific antibody 4D and primer pairs P1/ PD were used for PPV D subgrubing, while PPV-M (Marcus) subgrouping was conducted with M specific AL antibody and primer pairs P1/ PM. In Kayseri city center, 47 samples (89%) were found to be PPV infected. RT PCR results suggested that all positive samples are PPV-M. However, Of the 47 positive samples 33 reacted both against to PPM-D and PPV-M specific antibodies. Previous researches showed that some of this kind of ambiguous isolates could be PPV-T (Turkey). The disease were determined first time in a seven years old plum orchard in Yahyalı. The prevalence of PPV in Kayseri were found to be high suggesting that the disease in exist in Kayseri for a long time.

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Sharka disease , Plum pox virus , DASI , ELISA , RT , PCR , Stone fruits

Erciyes University Journal Of The Institute Of Science an‎d Technology

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