Author/Authors :
AYDIN, Murat Atatürk Üniversitesi - Ziraat Fakültesi - Tarla Bitkileri Bölümü, Turkey , SAĞSÖZ, Sevim Atatürk Üniversitesi - Ziraat Fakültesi - Tarla Bitkileri Bölümü, Turkey , HALİLOĞLU, Kamil Atatürk Üniversitesi - Ziraat Fakültesi - Tarla Bitkileri Bölümü, Turkey , TOSUN, Metin Atatürk Üniversitesi - Ziraat Fakültesi - Tarla Bitkileri Bölümü, Turkey
Title Of Article :
Factors Affecting Wheat Mature Embryo Culture
Abstract :
This study was carried out to determine the factors affecting wheat mature embryo culture. Effects of genotype, solidifying agents, auxin type and doses were found highly significant on formation of callus, embryogenic callus and regeneration capacity. Dicamba as an auxin type was better than 2,4-D and picloram and phytagel as a solidifying agent was better than agar in wheat mature embryo culture. The highest formation of callus, embryogenic callus and regeneration capacity were observed in medium containing 4 mg/l dicamba solidified with phytagel. Regeneration medium influenced significantly regeneration capacity. The highest regeneration capacity (R1) was observed in medium containing 1 mg/l 2,4-D + 0.5 mg/l BAP.
NaturalLanguageKeyword :
Genotype , auxin , solidifying agent , regeneration medium , Triticum aestivum
JournalTitle :
Journal Of Agricultural Faculty Of Atatürk University
