Youmbi, E. University of Yaounde I - Faculty of Sciences - Laboratory of Biotechnology and Environment, Unit of Physiology and Plant Breeding, Cameroon , Youmbi, E. Centre Africain de Recherche sur Bananiers et Plantains (CARBAP), Cameroun , TCHANA NYAMBEU, M. N. University of Yaounde I - Faculty of Sciences - Laboratory of Biotechnology and Environment, Unit of Physiology and Plant Breeding, Cameroon , NGAHA, D. Centre Africain de Recherche sur Bananiers et Plantains (CARBAP), Cameroun , FONBAH, C. Centre Africain de Recherche sur Bananiers et Plantains (CARBAP), Cameroun

In vitro PROLIFERATION ABILITY OF AXILLARY BUDS IN Musa spp

28909

Tissue culture method has always considered the apical bud as the initial explant for micropropagation of plantain until recently where it was shown with the variety Big Ebanga that axillary buds could equally serve as initial explant. As axillary buds have shown mass propagation abilities in Big Ebanga, this explant is tested in other banana varieties to confirm performances already observed. Axillary buds and shoot tips excised from : Pisang Mas (AA), Grande Naine (AAA), Batard and French Clair (AAB), CRBP 39 (AAAB) and Pelipita (ABB) varieties were used as explants. The proliferation rate of Axillary and apical buds and other growth parameters were measured. Results showed that no significant differences were observed between the two types of buds after four to five sub cultures in all the varieties except for CRBP 39 where the axillary bud exhibits a higher proliferation rate. Concerning the frequency of leaf emission, the diameter and the height of the pseudostems, no significant differences were obtained between plants derived from the two types of buds during the weaning and hardening phases. In conclusion, axillary buds proliferated for all varieties tested regardless of the genomic group. Plantlets obtained from the two types of buds showed similar performance during the weaning and hardening phases. The axillary bud can thus be used as initial explant for micro propagation in Musa sp.

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Musa sp. , micropropagation , axillary buds , shoot tips , genomic group

Agronomie Africaine

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