Kaygusuz, Rakibe Cumhuriyet Üniversitesi - Tıp Fakültesi - Mikrobiyoloji Anabilim Dalı, Turkey , Çelik, Cem Cumhuriyet Üniversitesi - Tıp Fakültesi - Mikrobiyoloji Anabilim Dalı, Turkey , Çabuk, Aslı Cumhuriyet University - School of Medicine - Department of Microbiology, Turkey , Bakıcı, Mustafa Zahir Cumhuriyet University - School of Medicine - Department of Microbiology, Turkey

A retrospective investigation of extended spectrum beta lactamase production in gram negative bacteria strains isolated from inpatients of an university hospital.

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production status of Gr (-) bacillus that were isolated from the cultures of several clinical specimens from the inpatients of our hospital in the years 2005 and 2006. Methods. Gram negative bacillus such as Escherichia coli (E.coli), Klebsiella pneumonia (K. pneumonia), Klebsiella oxytoca (K. oxytoca) were isolated from the cultures of the specimens of blood, urine, wound swab, sputum, trans tracheal aspirate material (TTA), vaginal swab, abscess, cerebrospinal fluid, peritoneal paracentesis fluid and catheter tip of 925 patients in the year 2005 and 1184 patients in the year 2006. Becton Dickinson Phoenix 100 model full automatic systems were used to identify bacteria and their sensitivity/resistance profile and to investigate whether these isolated gr(-) bacteria produce ESBL or not. Results. 2109 Gram negative bacterial strains (E.coli, K. pneumonia, K. oxytoca) were investigated for ESBL in two-year period (2005-2006). ESBL was positive in 591 of them. 273 out of 925 gram negative strains (30%) showed ESBL positivity in 2005 while 318 out of 1184 gram negative strains (27%) showed ESBL positivity in 2006. There was not a significant difference between the years 2005 and 2006 in terms of ESBL positivity (p 0.05). Two hundred and eleven (30%) strains of 709 E.coli, 44 (27%) strains of 164 K.pneumoniae and 18 (35%) strains of 52 K.oxytoca were found to be ESBL positive in the year 2005. Two hundred thirty seven (29%) strains of 831 E.coli, 54 (21%) strains of 253 K.pneumoniae and 27 (27%) strains of 100 K.oxytoca were found to be ESBL positive in the year 2006. There were no significant differences in the ratios of E.coli, K.pneumoniae and K.oxytoca between the years 2005 and 2006 in terms of ESBL positivity. Conclusion. Although there were no significant differences in the ratios of ESBL positivity between the 2005 and 2006 years, ESBL positivity still remains to be an important problem for our hospital when these high ratios are considered.

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Gram negative bacteria , extended spectrum beta lactamase , nosocominal infection

Cumhuriyet Medical Journal

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