Author/Authors :
Tetik, Şermin Cyprus International University - Faculty of Pharmacy - Department of Biochemistry and Molecular Biology, Turkey , Özaltun, Kaysu Marmara University - Pharmacy of Faculty - Department of Biochemistry, Turkey , Özakpınar, Özlem Marmara University - Pharmacy of Faculty - Department of Biochemistry, Turkey , Dışbudak, Murat Marmara University - Pharmacy of Faculty - Department of Biochemistry, Turkey , Rizaner, Nahit Cyprus International University - Faculty of Pharmacy - Department of Biochemistry and Molecular Biology, Turkey , Yavuz, Şule Marmara University - School of Medicine - Department of Rhomatology, Turkey , Yardımcı, K. Turay Cyprus International University - Faculty of Pharmacy - Department of Biochemistry and Molecular Biology, Turkey
Abstract :
Aim. Behcet’s Disease is a chronic, systemic inflammatory disease. Oxidative stress related to plasma protein modifications may have an important role in the progression of Behcet’s Disease. In this study the effect of oxidative damage during the course of Behcet’s disease on plasma proteins and the effect of colchicine and anti-aggregating agents on prevention or depression of oxidative damage was evaluated by plasma protein characterization and quantitation. Methods. 45 Behcet’s Disease and 40 control subjects were included in this study. Study groups are determined as Group I: in inactive stage receiving no drugs, Group II: active stage receiving only colchicine (1.0-2.0 mg/day), and Group III: in active stage receiving colchicine and antiagregating agent (acetyl salycilic acid 100 mg/day). Plasma total protein, plasma carbonyl content, plasma total thiol levels were determined spectrophotometrically. Erythrocyte sedimentation rate (ESR) and C- reactive protein (CRP) were evaluated by conventional methods. The protein peak areas were analyzed through high pressure liquid chromatography (HPLC). Results. Plasma protein levels were significantly higher in Group I and Group II compared to controls. Plasma carbonyl levels were higher in Group I, II and III than the control groups. After colchicine ve antiaggregating agent treatment in patient groups plasma carbonyl and were found significantly lower whereas thiol levels were found significantly higher in Group III than the other patient groups. Respons of plasma CRP and ESR levels to treatment in Group III were significantly positive compared to the other groups. Plasma protein fragmantation was not dedected in Behcet’s diseases. Conclusion. The plasma protein data which developed upon oxidative stress is very valuable in physiopathology of Behcet’s Disease. When the relation between severity of the disease and the extent of oxidative stress is considered, multicentral and longterm clinical follow ups could be proposed.
