مرکز منطقه ای اطلاع رساني علوم و فناوري - Investigation of the Hypoglycemic and Antioxidant Effects of Lycopene in Chlorpyrifos-Ethyl Treated Diabetic Rats

Abstract :

The present study was carried out to investigate the antioxidant and hypoglycemic effect of lycopene (L) on chlorpyrifos-ethyl (CP) induced oxidative stress and hyperglycemia in normal and diabetic rats. Wistar albino 56 adult rats were used. Rats were randomly divided into eight groups, n=7. The groups were called as Group 1 control (C) (0.5 ml corn oil); Group 2 diabetes (D) (50 mg/kg STZ); Group 3 CP (2.7 mg/kg); Group 4 L (10 mg/kg); Group 5 D+CP; Group 6 D+L; Group 7 L+CP; Group 8 D+L+CP. Rat weight gains and fasting blood glucose were measured every week for 4 weeks. At the end of the study, malondialdehyde (MDA) and glutathione (GSH) were assessed in whole blood. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), insulin, acetylcholinesterase (AChE) and nitric oxide (NO) were determined in plasma. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, tumor necrosis factor-α (TNF-α) and total antioxidant capacity (TAC) were assessed in serum. Treatment of rats with of streptozotocin (STZ) and CP were showed that decreased in plasma insulin level while fasting blood glucose and serum glucose increased compared to the control group. Same treatments were revealed increased in MDA while TAC and GSH levels were decreased with a consistently significant decreased in the activity of antioxidative enzymes. Moreover administration of lycopene to rat in D group was found that decrease in MDA while TAC and GSH levels were increased with a consistently increased in the activity of antioxidative enzymes. This study suggests that lycopene acts as a safe and efficient choice in helper to treatment of diabetes and chlorpyrifos-ethyl induced hyperglycemia and their complications.