AVCI, Gülcan Afyon Kocatepe University - Faculty of Veterinary Medicine - Department of Biochemistry, TURKEY , BÜLBÜL, Aziz Afyon Kocatepe University - Faculty of Veterinary Medicine - Department of Physiology, TURKEY , ÖZDEMİR, Mehmet Afyon Kocatepe University - Faculty of Veterinary Medicine - Department of Pharmacology and Toxicology, TURKEY , GÖKSU EROL, A.Yasemin Akdeniz University - Faculty of Medicine - Department of Histology and Embryology, TURKEY

The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats

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The purpose of this study was to investigate the effects of omega-3 (O-3) and sesame oil (SO) on cyclosporine-A (CsA) induced immunosuppressive and oxidant effects in rats. The study was performed on seven groups for 15 days using Wistar Albino rats (n=7),1) Control: physiological saline was given by gastric gavage, 2) CsA: CsA was administered (15 mg/kg/day) subcutaneously (sc),3) CsA+SO: CsA (15 mg/kg/day; sc) and SO (1 ml/kg, gavage) were given, 4) CsA+O-3: CsA, (15 mg/kg/day; sc) and O-3 (100 mg/kg, gavage) were administered, 5) CsA+SO+O-3: CsA (15 mg/kg/day; sc), SO (1 ml/kg, gavage) and O-3 (100 mg/kg, gavage) were given at the same time, 6) SO: only SO (1 ml/kg; gavage) 7) O-3: only O-3 (100 mg/kg, gavage). 24 h after the last treatments animals were sacrificed. Blood, livers and kidneys were collected for biochemical analysis. There was no difference among all groups in terms of nitric oxide (NOx). Changes in the IL-1α, IL-1β levels were insignificant all other groups compared to CsA group. Nonetheless IFN-γ levels were increased in all groups except SO, O-3 groups. Changes in the level of MDA, GSH, SOD and CAT in both kidney and liver tissues were negligible in the CsA group compared to control group. MDA, GSH and SOD levels in the kidney tissue were determined higher in the CsA groups than CsA+O-3 group, nevertheless changes in the liver were insignificant. Given these findings, use of SO and O-3 with CsA may be a new approach for preventing CsA- induced oxidative stress in kidneys.

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Antioxidants , Cyclosporine , A , Cytokines , Omega , 3 , Sesame Oil

Kocatepe Veterinary Journal

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