Author/Authors :
Çoban, Zehra Dilşad Gülhane Askeri Tıp Akademisi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Avcu, Ferit Sağlık Bilimleri Enstitüsü Araştırma ve Geliştirme Merkezi - Gülhane Askeri Tıp Akademisi, Turkey , Ural, Ali Uğur Sağlık Bilimleri Enstitüsü Araştırma ve Geliştirme Merkezi - Gülhane Askeri Tıp Akademisi, Turkey , Güran, Şefik Gülhane Askeri Tıp Akademisi - Tıbbi Biyoloji Anabilim Dalı, Turkey
Title Of Article :
The cytotoxic effect of docetaxel on Ig G plasma cell leukemia cell line ARH-77 as a chemotherapeutic agent
Abstract :
Aim.: Docetaxel causes microtubule stabilization during mitotic spindle organizing in cell and forces the cell to go apoptosis. Docetaxel is widely used in breast, prostate and bladder cancer. The potential cytotoxic effect of docetaxel, a chemotherapeutic agent which is used in various cancers, on Ig G plasma cell leukemia cell line (ARH-77- ATCC No: CRL-1621) was investigated in this study. Methods. The cytotoxic effect of docetaxel on ARH-77 cell line was evaluated at different concentrations namely 0.1-1-10-50-100 umol/L by using XTTtest. Result. It was shown that, docetaxel had significant cytotoxic affect at higher concentrations on ARH-77 cell line which forms a sample of human Ig G plasma cell leukemia. Conclusion. The data represent that the lethal dose of docetaxel in ARH-77 cell line is at the concentration of 50 umol/L. Our results suggest that docetaxel could be useful in the treatment of plasma cell leukemia of rare cases associated with poor prognosis. But further tests are needed to use this drug in plasma cell leukemia.
NaturalLanguageKeyword :
Docetaxel , ARH , 77 , mitotic catastrophe , plasma cell leukemia , cytotoxic effect
JournalTitle :
Cumhuriyet Medical Journal
