Aydıner, Çağatay Yalçın S. B. Şevket Yılmaz Eğitim ve Araştırma Hastanesi - Çocuk Cerrahisi Kliniği, Turkey , Pul, Mehmet Trakya University - Faculty of Medicine - Çocuk Cerrahisi Anabilim Dalı, Turkey , İnan, Mustafa Trakya University - Faculty of Medicine - Çocuk Cerrahisi Anabilim Dalı, Turkey , Bilgi, Selçuk Acıbadem Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, Turkey , Çakır, Erol Trakya Üniversitesi - Tıp Fakültesi - Biyokimya Anabilim Dalı, Turkey

Can n-acetylcysteine play a role on preventing tissue damage on experimental testicular torsion

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Aim. Testicular torsion, which is in the form of ischemia-reperfusion, is an important cause of acute scrotum. The morbidity could be decreased by an early and successful detorsion procedure. The aim of this study was to determine the effects of N-acetylcysteine on both testes in experimental rat model of unilateral testicular torsion and detorsion. Method. In this study, 32 prepubertal male albino Wistar rats were used. The rats were divided into four groups as control, torsion, detorsion and detorsion/N-acetylcysteine in order to determine the malondialdehyde level and histopathological damage. Rats in the control group were killed after the testes were removed without torsion. Right testes of the rats underwent four hours of torsion in other groups. N-acetylcysteine was injected intravenously 15 min before the detorsion in detorsion/N-acetylcysteine group. Reperfusion was continued for four hours after detorsion in detorsion and detorsion/N-acetylcysteine groups. Results. At the end of the experiment, both testes were studied for their malondialdehyde levels and histopathological evaluations in all groups. The malondialdehyde results were compared using the Kruskal-Wallis analysis of variance and Mann Whitney U-tests with p 0.05 considered to be significant. In all groups, malondialdehyde levels of both testes were found to be higher than those in the control group (p 0.05). There was no significant difference between detorsion/N-acetylcysteine and detorsion groups in malondialdehyde levels on both sides (p 0.05). After the procedure, interstitial hemorrhage and irregularity in tubule structures were observed on ipsilateral side in all groups apart from the control group. However, on the contralateral side, there were no significant histopathological differences except interstitial edema and a few germ cell desquamations in some cases. Conclusion. In conclusion, intravenous N-acetylcysteine administration before the detorsion in the model of experimental testicular torsion has no effect on the histopathological damage and malondialdehyde level.

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Testicular torsion , ischemia , reperfusion injury , N , acetylcysteine

Cumhuriyet Medical Journal

471