مرکز منطقه ای اطلاع رساني علوم و فناوري - Effects of Pomegranate (Punica granatum L.) on Paracetamol Induced Acute Hepatic Damage in Mice

Abstract :

The most common cause of acute liver failure has been reported as paracetamol toxicity. There are some drugs that can be used as an antidote for acetaminophen toxicity, but in some cases these drugs are ineffective. The purpose of this study is to identify protective and side effects of lyophilized extract’s from Punica granatum L. in acute liver injury model. In our study, 80 Swiss Albino mice were used. The research was conducted on two main groups. The mice in the side effect group were given pomegranate extracts at 100, 200 and 400 mg/kg doses. The trial group were administered mentioned doses of extracts and a single dose of paracetamol simultaneously. The results gained from biochemistry analysis demonstrated that lyophilized extract given along with paracetamol caused a significant decrease in serum AST, ALT, TP and TG levels. The pomegranate extract given to the experiment group led to a decrease in RBC, HB, HCT and LYMP levels. Histopathological evaluation demonstrated that the extract alone may cause liver injury ranging from mild to moderate. Yet the extract given simultaneously with paracetamol particularly may have a protective effect on liver in a certain dose of 200 mg/kg according to biochemistry results and in a certain dose of 400 mg/kg according to histopathological analysis. Measured AoA levels emphasize the antioxidant activity of the extract for trial group. The results obtained from the side effect group have emphasized possible reducing effect of antioxidant activity of lyophilized extract in mice.