Author/Authors :
tirpan, mehmet borga ankara university - faculty of veterinary medicine - department of reproduction and artificial insemination, Turkey , olğaç, kemal tuna ankara university - faculty of veterinary medicine - department of reproduction and artificial insemination, Turkey , gürler, hakan university of veterinary medicine hannover foundation - clinic for cattle, GERMANY , kaya, ufuk ankara university - faculty of veterinary medicine - department of biostatistic, Turkey
Abstract :
Equilibration is the one of the important steps of semen cryopreservation and it is the stage in which semen is cooled and phase changes occur. The condition of equilibration is mostly regarded; and success of the equilibration conditions depend on empirical experiences. The aim of this study was to investigate the effects of three different equilibration methods on post-thaw total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI). In each of 4 bulls; two ejaculates were split into three aliquots, diluted by Andromed® and equilibrated for 24h at 4°C in Drawed Straw (Experiment 1), Cups (Experiment 2) and Shaker (Experiment 3). After thawing, sperm quality was determined by examination of TMOT, PMOT, VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF, PMAI and DFI. There were no differences (p 0.05) depending on equilibration conditions for TMOT, PMOT, VSL, VAP, BCF as kinetic parameters and for PMAI and DFI as morphological parameters. The highest VCL value was observed in experiment 3, when the highest LIN, STR, WOB and ALH values were observed in experiment 2 (p 0.05). The results show that changes in equilibrating condition have no detrimental effect on post-thaw bull semen quality. In addition, it can be said that equilibrating bull semen in a shaker presents some kinetic benefits in contrast with cup or straw equilibration methods.
NaturalLanguageKeyword :
Bull Semen , CASA , Equilibration , Equilibration Condition , Flow Cytometry
