مرکز منطقه ای اطلاع رساني علوم و فناوري - The scolocidal effect of propolis on protoscoleces and daugther cysts

Abstract :

Objective: Scolocidal elements are used in the aim of not scattering the protoscoleces’ outside the cyst during the treatment of cyst hydatid in our present-day. It is hoped from the scolocidal materials; besides its effect on protoscoleces, not being toxic, being easy practicable, not causing sclerozan cholanjit and if possible, penetrating in the daughter vesicules. In this research, the effect of propolis which is a bee product on protoscoleces were investigated. Methods: In our work, daughter vesicules and protoscoleces obtain from the liver of a patient during surgery and propolis from the Black Sea Region were used. Viability tests were done by using 0.1% eosine solution. After propolis disolved in dimethylsulfoxide, within the range of 50 mg/mL and 0.010 mg/mL using by 0.9% serum physiological solution 100 μl into each of the tube and in 1.,3.,5.,10.,20.,and 30. minutes the rates of viability of protoscolex (4000 protoscolex/mL) are fixed. The research were subjected to daughter vesicules that is 4-7 mm in diameters. The concentration of propolis at 0.80 and 3.12 mg/mL were investigated viability rates of protoscolex at different incubation time and whether the possibilty of penetration to the daughter vesicules. Results: It was seen that protoscolexs were effected by the Propolis at 0.100 mg/mL concentration in the first minute, at 0.050 mg/mL concentration in the 3. minutes, at 0.025 mg/mL concentration at 10. minutes and at 0.010 mg/mL concentration in 20. minutes. For protoscolex in the vesicules at 0.80 mg/mL concentration in 20. minute, in 3.12 mg/mL concentration in 10. minutes were found to be lethal. Conclusions: As the protoscolex could be killed by Propolis which penetrate to daughter cysts in a very short time as 10 minutes, we thing that it will be beneficial completing advanced studies.