Author/Authors :
Turgut, Nergiz Hacer Cumhuriyet University - School of Pharmacy - Department of Pharmacology, Turkey , Altun, Ahmet Cumhuriyet University - School of Medicine - Department of Pharmacology, Turkey , Kara, Haki Cumhuriyet University - School of Veterinary Medicine - Department of Pharmacology and Toxicology, Turkey , Tepe, Bektaş Kilis University - School of Science and Literature - Department of Molecular Biology and Genetics, Turkey , Ergül, Merve Cumhuriyet University - School of Pharmacy - Department of Pharmacology, Turkey , Ergül, Mustafa Cumhuriyet University - School of Pharmacy - Department of Biochemistry, Turkey , Tuncel, Necati Barış Canakkale University - School of Engineering - Department of Food Engineering, Turkey , Tuncel, Neşe Yılmaz Canakkale University - School of Engineering - Department of Food Engineering, Turkey
Abstract :
Objective: Gliomas are brain tumors with high morbidity and mortality. For the treatment of gliomas, it is important to develop new and powerful treatments that could complement existingclinical treatment. Lonicera caprifolium L. (L. caprifolium) has various uses in herbal traditionalmedicine. This study was conducted to determine the phenolic acid levels and DNA damage protection potential of L. caprifolium extract, and to explore the antitumor effect of the extract byinvestigating its toxicity on C6 rat glioma cell lines and normal L929 mouse fibroblast cell lines.We also aimed to investigate the antiangiogenic potential of the extract. Method: Phenolic acid content was determined by HPLC analysis. DNA damage protection potentialwas evaluated on pBR322 plasmid DNA. The effect of extracts on the proliferation of cancercells was evaluated by XTT assay. Antiangiogenic effect was determined with Chorioallantoicmembrane model. Results: The extract was found rich in vanillic acid (273.003 μg/g); while the amount of chlorogenic acid was almost at negligible level (0.028 μg/g). 0.005-0.05 mg / ml extract protected against the hazardous effects of UV and H2O2 in all DNA bands. The presence of the extract significantly reduced C6 cell proliferation compared to control (p 0.05). The extract had antiproliferative effect with a half maximum inhibition of concentration (IC50) value of 0.45 mg/ml. L. caprifolium extract in 10-6, 10-5 and 10-4 M concentrations caused antiangiogenic effect. Antiangiogenic scores of L. caprifolium were 0.6, 0.73 and 1.6, respectively. Conclusions: These results show that L. caprifolium has potential cytotoxic and antiangiogenic effect on C6 rat glioma cells and that the phenolic acid content of the plant may partially influence these activities.
NaturalLanguageKeyword :
Glioma , Lonicera caprifolium , angiogenesis , cytotoxic effect , phenolic acid
