Şahin-Bölükbaşı, Serap Cumhuriyet University - Faculty of Pharmacy - Department of Biochemistry, Turkey , Öztürk, Safiye Emirdağ Ege University - Faculty of Science - Department of Biochemistry, Turkey , Anıl, Hüseyin Ege University - Faculty of Science - Department of Biochemistry, Turkey , Siliğ, Yavuz Cumhuriyet University - School of Medicine - Department of Biochemistry, Turkey

Effects of egonol that isolated from seeds of Styrax officinalis (L.) on lipid peroxidation and antioxidant defense system in human erythrocytes

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Objective: The aim of the present study was to investigate the effects on antioxidant defense system in human erythrocytes exposed to egonol that isolated from seeds of Styrax officinalis L.Method: A solution of Egonol was added so that final concentrations of Egonol were 0.2, 0.4, 0.6,0.8, and 1 mg/ml and erythrocyte pellets were incubated for 15 minutes. Malondialdehyde (MDA)level, activities of glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD),glutathione-S-transferase (GST) glutathione were determined in hemolysis of human erythrocytes.Results: Activities of SOD and GST were markedly increased in erythrocyte of human bloodcompared to the control group (p 0.05) in all concentrations of egonol. This increase also dosedependent manner. The activities of SOD and GST enzymes were increased 30% and 70%, respectively, in 1 mg/ml egonol concentration. There were no statistically significant alterations in GPx and CAT enzyme activities. In addition, MDA level that is an indicator of lipid peroxidation was not found to be increased significantly. Conclusion: Those results revealed that egonol has no effect of enhancing free radical formation in erythrocyte thus also causes no increase lipid peroxidation was observed. It was observed that, egonol was increased SOD and GST enzymes activities and free radicals were disappeared by the antioxidant defense system so erythrocytes were preserved from the lipid peroxidation.

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Antioxidant defense system , egonol , human erythrocytes , Styrax officinalis L

Cumhuriyet Medical Journal

178