مرکز منطقه ای اطلاع رساني علوم و فناوري - THE EFFECTS OF DIFFERENT AUXIN TYPES AND ACTIVE CHARCOAL LEVELS ON PLANT GROWTH AND DEVELOPMENT AT THE ACCLIMIZATION STAGE IN DIFFERENT STRAWBERRY CULTIVARS

Abstract :

Camarosa, Chandler and Oso Grande strawberry cultivars reproduced with meristem culture were investigated in this study in order to identify the effects of different chemicals used as rooting medias on plant growth and development during the acclimization stage. In the trial, besides activated charcoal (1.0, 2.5 ve 5.0 g L^-1), IBA (0.1 mg L^-1 ve 0.4 mg L^-1) and NAA (0.1 mg L^-1 ve 0.4 mg L^-1) hormones were used. It was revealed that active charcoal usage at rooting stage had positive effect on plant growth and development during acclimization whereas NAA and IBA hormones had negative effect. Also, some differences were observed between NAA and IBA in terms of plant development, and the use of auxin at high concentrations (0.4 mg L^-1) decreased development further at acclimization process. In the research, no problem was observed during acclimization stage for each three active charcoal concentration tried. Plants rooted in active charcoal media had a very high survival rate determined after they had been transferred to soil and brought to planting stage, and there were no losses. However in plants which had been rooted in media containing IBA and NAA, the loss during transfer to terrain was very high, therefore it was revealed that they should not be used for micropropagation. At the end of the research, it was also determined that plants, which were reproduced by meristem culture, need at least 12 weeks from meristem growing and development stage to soil transferring stage, and 2 months of acclimization between soil transfer stage and planting stage.