EGESEL, Cem Ömer Çanakkale Onsekiz Mart Üniversitesi - Ziraat Fakültesi - Tarımsal Biyoteknoloji Bölümü, TURKEY , KAHRIMAN, Fatih Çanakkale Onsekiz Mart Üniversitesi - Ziraat Fakültesi - Tarımsal Biyoteknoloji Bölümü, TURKEY , ÇORBACIOĞLU, Nur Çanakkale Onsekiz Mart Üniversitesi - Ziraat Fakültesi - Tarımsal Biyoteknoloji Bölümü, Turkey

THE EFFECT OF GENERATION AND PARENTS ON ENDOSPERM PROTEIN RATIO AND CHANGE OF PROTEIN FRACTIONS IN MAIZE

33864

Among the quality traits of maize grain, protein ratio and protein quality are two main components. The objective of the study was to investigate changes of endosperm protein ratio and protein fractions in successive generations of different hybrid combinations having a high lysine mutant parent, opaque2 (o2). The study was carried out using seed samples generated at 2010 and 2011 growing seasons in Dardanos Research and Experiment Center of Çanakkale Onsekiz Mart University. To produce the plant material, an o2 line was pollinated with two different inbreds. The seeds of F0:1 and F1:2 generations from these hybrids, as well as selfed seeds from the parents, were used to detect protein ratio and protein fractions. The results showed that there were significant differences between parent lines and F1:2 seeds of hybrid combinations for endosperm protein ratios. Endosperm protein ratios ranged between 5.54% and 8.93%. There was a significant male parent effect on albumin + globulin and zein fractions as well as total protein ratio. Albumin + globulin fractions ranged between 3.46-8.71 mg/g. Lower values were detected for zein and total protein ratios in F1:2 seeds as compared to the seeds of F0:1 generation. In terms of zein fractions the range was between 16.9 and 61.0 mg/g.

150

Albumin , Globulin , Protein , Zea mays

Anadolu Journal Of Agricultural Sciences

156