Deligöz, İlyas Karadeniz Tarımsal Araştırma Enstitüsü - Bitki Sağlığı Bölümü, Turkey , Değirmenci, Kemal Zirai Mücadele Merkez Araştırma Enstitüsü - Bitki Hastalıkları Bölümü, Turkey , Sökmen, Miray Arlı Ondokuz Mayıs Üniversitesi - Ziraat Fakültesi - Bitki Koruma Bölümü, Turkey

Determination of Plum pox virus, the causal agent of Sharka Disease, in Samsun Province

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Plum pox virus (PPV) is one of the most destructive and serious viral agent of stone fruits such as plum,peach, nectarine, apricot, almond, sweet and sour cherries. PPV strains have been classified into at leasteight sub-groups. PPV has been detected in some orchards in different geographical regions of Turkey including Marmara, Central Anatolia, Aegean and Mediterranean, and the strains of PPV and theirdistributions have been determined. To date, there is no report on the presence of PPV in the Black Sea Region. PPV-like fruit and leaf symptoms were noticed in four stone fruit orchards in Canik district ofSamsun province in July 2013, and a total of 22 samples were taken from nine apricot, six nectarine, fourplum and three peach trees. Only one plum and two apricot trees were found to be infected by DAS-ELISA using virus-spesific polyclonal antiserum and RT-PCR method using PPV-specific primers. The partial nucleotide sequences of coat protein (CP) region of plum and apricot isolates were obtained.Nucleotide BLAST analysis showed that plum and apricot isolates had the highest (99%) sequencesimilarity at nucleotide level with PPV-AbTk isolate (PPV-T strain) found on apricot trees in Ankara province and the PPV Abricotier Turquie isolate. This study revealed that the occurrence and distribution of PPV infection in stone fruit orchards needs to be investigated in the Black Sea Region in detail.

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CP , Plum , Apricot , PPV , T strain

Anadolu Journal Of Agricultural Sciences

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