مرکز منطقه ای اطلاع رساني علوم و فناوري - The effect of temperature and host type on development of entomopathogenic nematode Heterorhabditis bacteriophora (Poinar, 1975) (Nematoda: Heterorhabditidae)

Abstract :

Experiments were conducted at 15, 24 and 30 °C. Late instar larvae of Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae), Achoria grisella (Fabricius, 1794) (Lepidoptera: Pyralidae) and Ephestia kuehniella (Zeller, 1879) (Lepidoptera: Pyralidae) were used as host. Every host exposed to 50infective juveniles of Heterorhabditis bacteriophora (Poinar, 1975). Results indicate that H. bacteriophora killed all host larvae at these three temperatures. For all host species, host death time was longer at 15 °C than at 24 and 30 °C, whereas there was no significant difference among host deathtimes at 24 and 30 °C. Host species did not change the host death times. Penetration efficiency of H. bacteriophora was affected by temperature and host types. Number of H. bacteriophora penetrated to host larvae was significantly greater at 24 and 30 °C than at 15 °C for all tested host. For all testedtemperatures, significantly greater numbers of H. bacteriophora penetrated to the G. mellonella than had A. grisella and E. kuehniella. Temperature significantly affected the time of the emergence ofinfective juveniles from the host cadaver, but host types are not. For all tested host species the latestemergence was determined at 15 °C. Infective juvenile production was significantly affected bytemperature and host species. The highest infective juvenile production was observed at 30 °C in G. mellonella whereas E. kuehniella had the lowest one at 15 °C.