Author/Authors :
Kaya, Mustafa Oğuzhan Siirt Üniversitesi - Veteriner Fakültesi - Temel Bilimler Bölümü, Biyokimya Anabilim Dalı, Turkey , Yerlikaya, Emrah Siirt Üniversitesi - Siirt Sağlık Yüksekokulu - Beslenme ve Diyetetik Bölümü, Turkey , Özyazıcı, Mehmet Arif Siirt Üniversitesi - Ziraat Fakültesi - Tarla Bitkileri Bölümü, Turkey , İrak, Kıvanç Siirt Üniversitesi - Veteriner Fakültesi - Temel Bilimler Bölümü, Biyokimya Anabilim Dalı, Turkey
Title Of Article :
In vitro effects of some forage industry chemicals on hyaluronidase
Abstract :
This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10^-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10^-2 mM.
NaturalLanguageKeyword :
Fertilizer , Hyaluronidase , Inhibition , Artificial insemination , Forage
JournalTitle :
Anadolu Journal Of Agricultural Sciences
