مرکز منطقه ای اطلاع رساني علوم و فناوري - Determining of resistance mechanism against abiotic stress factories in native walnut variety (Juglans regia L.)

Abstract :

This study investigated the reactions of native walnut genotype (Juglans regia L.) against different abiotic stress factors. For this purpose, 5-6 leaves seedlings grown in the plastic pots having the mixture of peat, garden soil and sand (2:2:1) were treated with salinity (75, 150 and 225 mM NaCl), heavy metals (2 mg/L FeCl3, NiCl2 and ZnCl2), lime (2 g/L CaCO3), pollution (2 g/L factory flue dust) and drought (50%) for six weeks. After the treatments, photosynthetic pigment, proline, total soluble protein, peroxidation level (MDA-malondialdehyde), H2O2, APx, CAT and SOD activities in the leaf samples were analysed. Results showed that chlorophyll b and total chlorophyll and carotenoids were highest for the lime and heavy metals treatments, whereas total soluble protein was highest for iron, zinc and factory flue dust treatments. CAT activity was highest for zinc, nickel and lime treatments,while proline, H2O2 concentration, APx and SOD activity were highest for all treatments. Lipid peroxidation level (malondialdehyde-MDA) starch showed an increase only with the lime treated leaves but it was lower with other treatments. In conclusion, the results indicate that the studied walnut genotype is much tolerant for heavy metal toxicity while it is susceptible to salinity concentrations and drought. In addition, walnut variety shows medium tolerance against the factory flue dust and lime.