altop, emine kaya ondokuz mayıs üniversitesi - ziraat fakültesi - bitki koruma bölümü, Samsun, turkey , mennan, hüsrev ondokuz mayıs üniversitesi - ziraat fakültesi - bitki koruma bölümü, Samsun, turkey , işık, doğan erciyes üniversitesi - ziraat fakültesi - bitki koruma bölümü, Kayseri, turkey

PCR‐based detection of resistance to ALS‐inhibiting herbicides of Bifora radians Bieb. (Bifra) in Wheat Fields

34021

Weeds are one of the most important yield limiting factors in wheat which makes an effective weed control an integral part of production. Due to intensive herbicide use in wheat growing areas in Turkey is resistance problems in some important weed species. There are some observations concerning the efficacy failures of some ALS inhibitor herbicides on some important weed species, such as Bifora radians. With this aim weed seeds will be collected from different wheat growing provinces in Central Anatolia and Black Sea Region of Turkey, in total from 1371 different wheat fields. After initial screening for resistance in pot experiments, biotypes that are supposed to be resistant will be submitted to bioassays to determine the resistance grades. Some accession exhibited the highest survival to treatment with field rates of these herbicides. The Weibull dose-response curve, an asymmetric sigmoid curve, was fitted to data to obtain ED50 in R program. Twenty-two of B. radians accessions tested did not controlled by ALS inhibitor and they were accepted as a resistant population. Based on the results of bioassays, resistant biotypes tested by means of molecular methods. The gene region of resistant biotypes was determined through sequence analyses and mutation wasn t found.

153

Bifora radians. ALS inhibitor. herbicides. Resistance. Bioassay. Molecular

Anadolu Journal Of Agricultural Sciences

163