arslan, fatma nur university of karamanoğlu mehmetbey - faculty of science - department of chemistry, Karaman, Turkey , akin, gönül university of karamanoğlu mehmetbey - faculty of science - department of chemistry, Karaman, Turkey , yilmaz, ibrahim university of karamanoğlu mehmetbey - faculty of science - department of chemistry, Karaman, Turkey

PHYSICOCHEMICAL CHARACTERISTICS, PESTICIDE RESIDUE AND AFLATOXIN CONTAMINATION OF COLD PRESSED PUMPKIN SEED (Cucurbita pepo L.) OILS FROM CENTRAL ANATOLIA REGION OF TURKEY

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In this study, physicochemical characteristics, pesticide residues and aflatoxin contaminations of cold pressed pumpkin seed (Cucurbita pepo L.) oils cultivated in four different central Anatolia regions of Turkey, were investigated. Lab-scale screw press machine was used to produce cold pressed pumpkin seed oils and the oil contents were found between 42.8%−47.4% for naked seeds. The physicochemical characteristic (refractive index, viscosity, color value, triglyceride profile analysis, peroxide value, iodine value, free fatty acid, saponification number, unsaponified matter, specific extinction values at 232 and 270 nm) of cold pressed oils were determined by using different analytical techniques. The results showed that there was a non-significant difference between cold pressed pumpkin seed oils from different regions, in terms of physicochemical characteristics. The contents of pesticide residue and aflatoxin B1, B2, G1 and G2 contamination were determined by using validated UHPLC-MS/MS method. The chlorpyrifos pesticide residue was detected under the limit value declared by official authorities for the quality assessment of edible oils. Aflatoxins weren’t detected in any of studied pumpkin seed oils. Therefore, the study provides important data for the cold pressed pumpkinseed oils, and proves that screw-pressed pumpkinseed oils are high quality, nutritious and valuable oils with higher levels of consumer acceptability.

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Cucurbita pepo L. , Cold pressed oil , Physicochemical characteristic , UHPLC , MS , MS

Anadolu University Journal of Science and Technology. A : Applied Sciences and Engineering

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