Author/Authors :
BILGE-DAGALP, Seval University of Ankara - Faculty of Veterinary Medicine - Depertment of Virology, Turkey , GUNGOR, Elvin University of Ankara - Faculty of Veterinary Medicine - Depertment of Virology, Turkey , DEMIR, Ayşe Başak University of Ankara - Faculty of Veterinary Medicine - Depertment of Virology, Turkey , PINAR-MUZ, Dilek University of Mustafa Kemal - Faculty of Veterinary Medicine - Depertment of Virology, Turkey , YILMAZ, Volkan Kafkas University - Faculty of Veterinary Medicine - Department of Virology, Turkey , OGUZOGLU, Tuba Çigdem University of Ankara - Faculty of Veterinary Medicine - Depertment of Virology, Turkey , ATASEVEN, Veysel Soydal Mustafa Kemal University - Faculty of Veterinary Medicine - Department of Virology, Turkey , ALKAN, Feray University of Ankara - Faculty of Veterinary Medicine - Department of Virology, Turkey
Abstract :
To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV- 4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.
NaturalLanguageKeyword :
Antibody , bovine herpesvirus type 4 , metritis , polymerase chain reaction , sequence analysis
