YÜKSEL, Deniz Ankara Üniversitesi - Faculty of Science - Biology Department, Turkey , AKÇELİK, Nefise Ankara Üniversitesi - Biotechnology Institute, Turkey , AKÇELİK, Mustafa Ankara Üniversitesi - Faculty of Science - Biology Department, Turkey

Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium

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The stj fimbrial operon harbors five genes, stjABCDE. The operon plays role in the persistence and virulence of S.Typhimurium in host systems. In this study, in vitro activation of the stj operon was achieved by transposon (TPOP) mutagenesis.The transposon insertion site was located within the cadC gene promoter in the stj operon-activated mutant strain MDN20.Comparative analysis of two-dimensional gel electrophoresis protein patterns of the wild type strain (S. Typhimurium LT2) and mutant strain MDN20 revealed that the CadC and StjB proteins were produced by mutant strain MDN20 but not by the wild type strain. These results were also confirmed by Maldi-TOF analysis. These findings indicate that cadC gene is a transcriptional activator of stj fimbrial operon in S. Typhimurium.

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S. Typhimurium , stj operon , cadC , transcriptional activator

Veterinary Journal of Ankara University

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