Author/Authors :
İNEGÖL, Emel Adnan Menderes University - Health Sciences Institute, TURKEY , TÜRKYILMAZ, Süheyla Adnan Menderes University - Faculty of Veterinary Medicine - Department of Microbiology, TURKEY
Abstract :
This study was conducted to determine staphylococcal cassette chromosome mec (SCCmec) types of methicillin resistant staphylococci (MRS) isolated from cattle and farm workers. A total of 145 subclinic mastitic bovine milk and 91 nasal swab samples (56 from cows and 35 from farm workers) were studied for presence of MRS. Methicillin resistance was detected by cefoxitin disc diffusion test. Bacterial identification and methicillin resistance was confirmed by 16S rRNA sequencing and mecA PCR, respectively. SCCmec types of these isolates were determined by multiplex PCR targeting for mec and ccr genes, associated IS element and plasmids. Among 236 total samples, 181 staphylococci, 59 of which were found as methicillin resistant were isolated.Fifty nine methicillin resistant isolates originated from 37 (62.7%) were bovine and 22 (37.3%) were human. Ten Staphylococcus species [S. haemolyticus (18), S. epidermidis (17), S. aureus (12), S. xylosus (4), S. cohnii (3), S. equorum (1), S. sciuri (1), S.succinus (1), S. pseudointermedius (1) and S. saprophyticus (1)] among 59 MRS were identified by 16S rRNA sequence analysis.SCCmec types of 2, 38, 18, and 1 MRS isolates were II, III, IV, and V, respectively. A total of 40 isolates including type II and III were hospital acquired whereas 19 isolates including type IV and V were community acquired. Almost all S. haemolyticus and S.aureus isolates carried hospital acquired type SCCmec. The similarity of SCCmec types between human and cattle isolates suggest evidence of transmission from animals to humans, or vice versa. Further studies are needed to establish clonal relationship of MRS isolated from bovine and farm workers with advanced molecular techniques.
NaturalLanguageKeyword :
Staphylococcus , methicillin , SCCmec , cattle , human
