Author/Authors :
ÖZTETİK, Elif Anadolu University - Science Faculty - Department of Biology, Turkey
Title Of Article :
A study of calcium release from rat liver microsomes by thapsigargicin induction
Abstract :
The primary aim of the study was to test the influences of thapsigargicin in releasing Ca^2+ from the intracellular Ca^2+ stores and comparing it with the effects of thapsigargin. In this study, the effect of tumour promoter thapsigargicin on intracellular Ca2+ has been described. Therefore, rat liver endoplasmic reticulum subcellular fractions (microsomes) were employed and Ca^2+ movements measured by spectrofluorimeter. Fluo-3 studies were used to follow Ca^2+ release in experiments with microsomes. When results are evaluated, it has been concluded that like thapsigargin, thapsigargicin has the ability of discharging the intracellular Ca^2+ stores, increasing the intracellular free Ca^2+ concentration ([Ca^2+]i) and being a potent and specific inhibitor of the ER Ca^2+ -ATPase.
NaturalLanguageKeyword :
Ca^2+ release , intracellular stores , SERCA ATPases , spectroscopy , thapsigargin (TG) , thapsigargicin (TGC)
JournalTitle :
Veterinary Journal of Ankara University
