AĞAOĞLU, Ali Reha Mehmet Akif Ersoy University - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey , KORKMAZ AĞAOĞLU, Özgecan Mehmet Akif Ersoy University - Faculty of Veterinary Medicine - Department of Animal Science, Turkey , KURAR, Ercan Selcuk University - Faculty of Veterinary Medicine, Advanced Technology Research and Application Center (ILTEK) - Department of Genetics, Turkey , GÜZELOĞLU, Aydın Selcuk University - Faculty of Veterinary Medicine, Advanced Technology Research and Application Center (ILTEK) - Department of Genetics, Turkey , KAYIŞ, Seyit Ali Selcuk University - Faculty of Agriculture - Department of Animal Science, Biometry-Genetics Unit, Turkey , ATLI, Mehmet Osman Dicle University - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey , SCHAFER-SOMI, Sabine University of Veterinary Medicine Vienna - Centre for Artificial Insemination and Embryo Transfer, Turkey , ASLAN, Selim Ankara Üniversitesi - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey

Expression of platelet activating factors and its receptor in cat uterus during early pregnancy

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Platelet activating factors (PAF1β, PAF1γ, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAF genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy. In conclusion, PAF genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy.

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PAF , cat , pregnancy , qRT , PCR.

Veterinary Journal of Ankara University

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