MUSAL, Bayazıt Adnan Menderes University - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey , BECERİKLİSOY, Hakkı B. Adnan Menderes University - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey , ULUTAŞ, Pınar. A. Adnan Menderes University - Faculty of Veterinary Medicine - Department of Biochemistry, Turkey

The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra

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In this study, rabbits were used as a model for canine pyometra to test the effects of preoperative therapy in the reduction of postoperative stress. Pyometra were induced in 18 healthy rabbits by the operative administration of Pasteurella multocida inoculum. Beginning on the third day after inoculation Group I (n=6) received enrofloxacin (2,5 mg/kg, s.c.), Group II (n=6) recieved enrofloxacin (2,5 mg/kg, s.c.) + vitamin C (100 mg, s.c.) injections for three days. Ovariohysterectomy were performed in groups I and II following this medical treatment. Group III (n=6) as the control group, did not recieve any medical therapy and were operated on the third day after inoculation. Blood samples were collected before inoculation, on the day of operation, 24th h, 72th h and at 7th day following the operations. Ceruloplasmin (CERU), malondialdehyde (MDA), vitamin C, haptoglobin (Hp) and serum amyloid-A (SAA) levels were analyzed. In the postoperative 72th h period mean CERU level of Group III were found significantly higher (p 0,05) than Group I and II. Mean MDA level were significantly higher in Group III on the day of operation compared to the treatment groups (p 0,05). SAA levels rised in all of the groups following inoculation, on the day of operation (p 0,05) which is followed by a gradual decline until the 7th day. Overall CERU and MDA levels tend to be higher in the control group compared to the treatment groups whereas Hp and SAA levels were not significantly different. These findings may indicate that preoperative therapy may reduce the oxidative stress mildly however it did not significantly alter the acute phase protein response definitively and a significant positive effect of both preoperative treatments compared to the control group could not be pronounced.

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Acute phase proteins , antioxidants , ovariohysterectomy , pyometra , rabbit.

Veterinary Journal of Ankara University

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