Author/Authors :
İnal, Emine Kübra Ankara Üniversitesi - Faculty of Science - Department of Chemistry, Turkey , Öz, Sevi Ahi Evran University - Faculty of Science and Arts - Department of Chemistry, Turkey , Atakol, Arda Ankara Üniversitesi - Faculty of Science - Department of Chemistry, Turkey , Akay, Mehmet Abdülkadir Ankara Üniversitesi - Faculty of Science - Department of Chemistry, Turkey , Atakol, Orhan Ankara Üniversitesi - Faculty of Science - Department of Chemistry, Turkey
Title Of Article :
Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples
Abstract :
3,5,4-trans-trihydroxystilbene (trans-resveratrol) is quite soluble in CH2Cl2 and CHCl3. Wine samples are shaken in and rinsed with CHCl3 to obtain fine extracts of trans-resveratrol. The obtained CHCl3 solutions were directly injected to HPLC system for analysis. Determinations were made in 60% MeCN: 40% EtOH, and by trans-resveratrol standard addition. The resolution is calculated to be about 1.0 in the chromatogram. In 9 wine samples produced in Turkey, resveratrol concentrations were detected in between 1.46 and 6.53 ppm. By consecutive extractions, extraction ratio of resveratrol was determined and by increasing the amount of the samples, accuracy of the method was tested. It was found that the recovery is about 100.42 %.
NaturalLanguageKeyword :
Resveratrol determination , wine , HPLC , liquid , liquid extraction
JournalTitle :
Süleyman Demirel University Faculty of Arts and Science Journal of Science
