Author/Authors :
Baş, Duygu Belkıs Ondokuz Mayıs Üniversitesi - Tıp Fakültesi - Farmakoloji Anabilim Dalı, Turkey , Bilge, Süleyman Sırrı Ondokuz Mayıs Üniversitesi - Tıp Fakültesi - Farmakoloji Anabilim Dalı, Turkey , Kesim, Yüksel Ondokuz Mayıs Üniversitesi - Tıp Fakültesi - Farmakoloji Anabilim Dalı, Turkey
Abstract :
In this study, the effects of non-steroidal anti-inflammatory drugs (NSAIDs) from different groups on various physiological mechanisms involving bladder muscle motility were investigated in isolated rat detrusor strips. For this purpose, in the presence of aspirin, indomethacin and ketoprofen the responses of isolated detrusor strips to carbachol, isoprenalin, and electrical field stimulation (EFS) were evaluated. For in vitro studies, bladder detrusor muscles were removed and placed in organ baths. At the beginning of experimental protocol, the responses of detrusor strips to carbachol, isoprenalin and EFS were taken as control responses and recorded. Subsequently, following 30 minutes incubation with NSAIDs these responses were recorded again. The changes that occur in bladder basal tone in the presence of each NSAIDs were also assessed. In the presence of aspirin basal tone did not change. Aspirin at low concentrations (10^-8M and 10^-7M) significantly reduced detrusor responses to carbachol. But, at the higher concentrations (10^-6-10^-4M) when compared with control responses there was no significant difference.Isoprenalin-evoked responses were not changed in the presence of aspirin (10^-7-10^-5M). Only, the lowest aspirin concentration (10^-7M) significantly reduced the high frequencies of EFS-evoked responses, but the low frequencies of EFS-evoked responses left unchanged. Furthermore, at the higher concentrations (10^-6M, 10^-5M) of aspirin did not change EFS-evoked responses. In the presence of indomethacin (10^-7-10^-5M) basal tone reduced and carbachol- and EFS-evoked responses did not change. The detrusor responses to isoprenalin significantly reduced dose-dependant way after incubation with indomethacin. Incubation with ketoprofen (10^-7-10^-5M), caused a fall in basal tone and significantly enhanced carbachol-evoked responses. Only the highest ketoprofen concentration significantly inhibited isoprenalin-evoked responses. Ketoprofen, at the doses used, did not change EFS-evoked responses. Our results show that; the effects of NSAIDs on isolated detrusor strips are mainly due to prostaglandin synthesis inhibition but contribution of other mechanisms can not be ruled out. Furthermore, effects of NSAIDs from different on these responses are different from each other.
