Author/Authors :
Altıkat, Sayit Atatürk Üniversitesi - Tıp Fakültesi - Biyokimya Anabilim Dalı, Turkey , Büyükokuroğlu, Mehmet Emin Atatürk Üniversitesi - Tıp Fakültesi - Farmakoloji Anabilim Dalı, Turkey , Çiftçi, Mehmet Atatürk Üniversitesi - Biyoteknoloji Uygulama ve Araştırma Merkezi, Turkey , Bakan, Nuri Atatürk Üniversitesi - Tıp Fakültesi - Biyokimya Anabilim Dalı, Turkey , Küfrevioğlu, Ömer İrfan Atatürk Üniversitesi - Fen Edebiyat Fakültesi - Biyokimya Anabilim Dalı, Turkey
Title Of Article :
In vitro effects of calcium gluconate, potassium chloride and sodium bicarbonate on human erythrocyte glucose 6-phosphate dehydrogenase
Abstract :
Effects of some electrolytes which are frequently used for treatment, on erythrocyte glucose 6-phosphate dehydrogenase enzyme (G6PD) activity were investigated in in vitro conditions. G6PD was purified by affinity chromatography. Enzyme activity was determined according to the Beutler method by using a spectrophotometer at 340 nm. According to the results of study. 50% inhibatör drug concertration values (I50) were 19.4 mM for calcium glukonat and 160 mM for sodium bikarbonat in in vitro study. There was not observed any inhibitör effect of potassium chloride on glucose 6-phosphate dehydrogenase activity. The result of this study revealed that calcium gluconate and sodium bicarbonate but not potassium chloride have significiant inhibititory effect on the activity of G6PD enzyme in in vitro.
NaturalLanguageKeyword :
Erythrocyte , Glucose 6 , phosphate dehydrogenase , In vitro , Calcium gluconate , Potassium chloride , Sodium bicarbonate
JournalTitle :
Journal Of Experimental and Clinical Medicine
