BULUT, Derya University of Yuzuncu Yil - Faculty of Agriculture - Department of Plant Protection, Turkey , SİPAHİOĞLU, Hikmet Murat University of Yuzuncu Yil - Faculty of Agriculture - Department of Plant Protection, Turkey , USTA, Mustafa University of Yuzuncu Yil - Faculty of Agriculture - Department of Plant Protection, Turkey

Performance Evaluation of Different Brands of Thermal Cyclers Used for Diagnostic Testing

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Reliability of PCR-based data between the thermal cyclers requires an assurance of assay reproducibility. In this work, for the validation and standardization of PCR, the precision of thermocyclers on PCR was measured in three commercial thermal cyclers. A reverse transcriptase polymerase chain reaction (RT-PCR) test was run in parallel to assess PCR results. In diagnostic testing, isolates of Apple mosaic virus (ApMV), Zuchini yellow mosaic virus (ZYMV), Cherry leaf roll virus (CLRV), and Potato spindle tuber viroid (PSTVd) were used as reference isolates. The cyclers mostly performed within the manufacturer’s specification. All thermal cyclers produced the correct target for the single-product PCR assay, although slight variation in yield was observed. For two replicates of each positive sample, repeatability was demonstrated for the tested three thermal cyclers. It was concluded that, irrespective of the make and model of thermal cycler, reproducibility of PCR products by means of RT-PCR was sufficiently acceptable to have similar results from independently in separate commercial thermal cyclers.

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Performance , Thermal cyclers , RT , PCR

Yuzuncu Yil University Journal Of Agricultural Sciences

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