Author/Authors :
JAM BARANDUZI, Ata university of mohaghegh ardabili - Faculty of Agriculture Sciences, اردبيل, ايران , SOFALIAN, Omid university of mohaghegh ardabili - Faculty of Agricultural Sciences - Dept of Agronomy and Plant Breeding, اردبيل, ايران , ASGHARI ZAKARIA, Rasool university of mohaghegh ardabili - Faculty of Agricultural Sciences - Dept of Agronomy and Plant Breeding, اردبيل, ايران , ASGHARI, Ali university of mohaghegh ardabili - Faculty of Agricultural Sciences - Dept of Agronomy and Plant Breeding, اردبيل, ايران , SHOKRPOUR, Majid university of tehran - Faculty of Agriculture and Natural Resources - Dep of Horticulture, تهران, ايران
Abstract :
Inter-Simple Sequence Repeat (ISSR) and ACID-PAGE analyses were used to evaluate genetic diversity and relationships of Aegilops species from North-West of Iran. Thirty-three accessions of six Aegilops species including [Ae. taucshii (D), Ae. cylindrica (CD), Ae. umbellulata (U), Ae. triuncialis (UC), Ae. biuncialis (UM) and Ae. crassa (DM)] were studied in ISSR study. Twelve of the accessions were employed in the ACID-PAGE study. Eleven ISSR primers produced 171 polymorphic bands, which were used to construct the dendrograms. In ISSR method, the studied Aegilops species were subgrouped and it is identified that the species with common genome standed in the same group. ACID-PAGE analysis revealed high rate of polymorphism among Aegilops accessions. Therefore, results showed that there was potencial genetic diversity in the Aegilops species from North-West of Iran, and it could be useful genetic stock in any breeding perspectives. Specially the intra specific genetic diversity could be the feasible genetic base for any selection activities.
NaturalLanguageKeyword :
ACID , PAGE , Aegilops , ISSR , Genetic diversity
