Author/Authors :
TÜRKÖZÜ, Didem Iğdır Üniversitesi - Tarla Bitkileri Bölümü, Turkey , YAŞAR, Fikret Yüzüncü Yıl Üniversitesi - Ziraat Fakültesi - Bahçe Bitkileri Bölümü, Turkey , ELLİALTIOĞLU, Ş. Şebnem Ankara Üniversitesi - Ziraat Fakültesi - Bahçe Bitkileri Bölümü, Turkey , YILDIRIM, Bünyamin Iğdır Üniversitesi - Tarla Bitkileri Bölümü, Turkey
Title Of Article :
Researches on in Vitro Propagation of Tarragon (Artemisia dracunculus L.)
Abstract :
Medicinal and aromatic plants, which are consumed as a dried or fresh tarragon plant (Artemisia dracunculus L.) through tissue culture propagation were studied. The tissue culture study on tarragon, explant type most suitable for in vitro propagation, carbonhydrate source, and sub-culture tried to determine the number. In this study, the optimal nutritional environment of different nutrient media tried and BA 1.8 μM, 0.3 μM NAA, 3 % sugar and 0.7 % agar supplemented Murashige and Skoog nutrient medium were determined. The highest growth rate of 92.0 % according to this value, Shoot tip x Granulated sugar were obtained from the combination. Three times were sub-culture, sub-cultures rosette and albino plant formation was observed. Addition of GA3 adversely affected the development. The longest roots were obtained from 0.5 mg /l IBA supplemented MS and ½ MS media (44±14.0 and 46±16.0 mm, respectively), hormone-free medium was determined to be shorter root lengths (34±11.0 and 35±08.0 mm ). Tarragon rooted plants were transferred to pots. Tarragon propagation by tissue culture was carried out successfully. Clonal micropropagation seen as a problem limiting the formation of albino plants and eliminate about it was that detailed studies should be performed.
NaturalLanguageKeyword :
Tarragon (Artemisia dracunculus L.) , Micropropagation , Medium , Shoot tip , Single node
JournalTitle :
Yuzuncu Yil University Journal Of Agricultural Sciences