Author/Authors :
DEMİRHAN, Elçin Yıldız Technical University - Department of Chemical Engineering, Turkey , KILIÇ APAR, Dilek Yıldız Technical University - Department of Chemical Engineering, Turkey , ÖZBEK, Belma Yıldız Technical University - Department of Chemical Engineering, Turkey
Title Of Article :
pH-STAT CALIBRATION FOR SESAME CAKE PROTEIN HYDROLYSIS BY ALCALASE
Abstract :
In the present study, pH-stat method was calibrated by TNBS and OPA reactions for control of hydrolysis of sesame cake protein by Alcalase. Hydrolysis experiments were conducted in a batch reactor at the temperature of 50°C for various pH values, and at pH 7.5 for the temperatures of 40°C and 60°C. The calibration was characterized by good linear correlations for both methods. The mean pK values of sesame cake protein hydrolysates were obtained as 6.88, 6.65 and 6.42 for 40, 50 and 60°C, respectively.
NaturalLanguageKeyword :
Sesame cake protein , hydrolysis , Alcalase , pH , stat , pK.
JournalTitle :
Sigma Journal Of Engineering and Natural Sciences
