Author/Authors
ARAL, Erinç Eskisehir Osmangazi University - Department of Histology and Embryology, Turkey , SUNAL, Emine Eskisehir Osmangazi University - Department of Biochemistry, Turkey , KILIÇ, Fatma Sultan Eskisehir Osmangazi University - Department of Pharmacology, Turkey , USLU, Sema Eskisehir Osmangazi University - Department of Biochemistry, Turkey , ÖNER, Setenay Eskisehir Osmangazi University - Department of Biostatistics, Turkey , EKER SARIBOYACI, Ayla Eskisehir Osmangazi University - Department of Histology and Embryology, Turkey , BURUKOGLU, Dilek Eskisehir Osmangazi University - Department of Histology and Embryology, Turkey , KILIÇOGLU, Murat Eskisehir Osmangazi University - Department of Histology and Embryology, Turkey
Title Of Article
GPSE (GRAPE SEED PROANTHOCYANIDIN EXTRACT) SIMVASTATIN AND SILYMARIN EFFECT ON HIGH CHOLESTEROL-FED ANIMALS
شماره ركورد
41974
Abstract
The present study was designed to test the hypothesis that cholesterol rich diet-induced hypercholesterolemia in rabbits can be reversed by long-term treatment of antioxidants (proanthocyanidin and silymarin) and antihyperlipidemic drug(simvastatin).In this study 35 adult New Zealand White (NZW) male rabbits were used and divided into five groups. The rabbits in Group 1(control) was fed with rabbit chow diet. Group 2 was fed with only cholesterol (2% w/w) rich diet to induce hypercholesterolemia. The other groups received GPSE 100mg/kg (Group 3), silymarin 10mg/kg (Group 4) and simvastatin10mg/kg (Group 5) with cholesterol in addition to rabbit chow diet. At the end of the experiment, the animals were sacrified and their aorta were excised for intimal lesion analysis. Vessells were dissected, trimmed, and then placed fixed in neutral formalin. All tissue sections were stained with hematoxylin and eosin(H E).Apoptotic process was detected by using TUNEL.Cholesterol treatment produced a sustainable state of atheromatous plaque formation In addition,only cholesterol rich diet-induced animals have shown a considerably high number apoptotic cells byusing TUNEL and plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found to be significantly high in this group (p 0.05). But simvastatin was significantly effective on thedecreased plasma lipid levels. Histomorphometric intimal lesion analysis of the aorta showed lessatheromatous plaque formation in simvastatin, GPSE and silymarin groups, respectively. Relaxationresponses were significantly decreased in cholesterol group, but simvastatin treatment and alsoantioxidants significantly increased ach responses to the control level.Cholesterol rich diet-induced hypercholesterolemia in rabbits partially was reversed by addingantioxidants prolonging its use. In addition simvastatin modulated lipid profiles.
From Page
101
NaturalLanguageKeyword
Atherosclerosis , Grape seed extract , Proanthocyanidin , Simvastatin , Silymarin
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
117
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
Link To Document