Association between Recurrent Pregnancy Losses and Mthfr C677t and A1298 and Pai-1 4g/5g Polymorphisms and Allele Frequencies in Muş

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Aim: In this study it was aimed to determine the association between plasminogen activator inhibitor type 1 gene (PAI-1) 4G/5G and methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms with recurrent pregnancy losses and the allele frequencies in Mus city. Materials and Methods: Genomic DNA was isolated from blood samples of 34 patients with recurrent pregnancy losses and 34 healthy controls. DNA was amplified with 4G and 5G specific primers for detection of PAI-1 gene 4G/5G polymorphism and PCR-RFLP technique was used to analyze MTHFR gene C677T and A1298C polymorphisms. Products were assessed with UV transilluminator by being exposed to agarose gel electrophoresis. Results: The frequencies of the PAI-1 gene 4G/5G and MTHFR gene C677T and A1298C alleles in the patient group were similar to control group. According to the genotype distribution of PAI-1 gene 4G/5G and MTHFR gene A1298C polymorphisms there was no significant difference between patient and control group. MTHFR gene 677 (TT) homozygote mutation has been detected only in patients and (CT) heterozygote mutation only in the control group, despite a statistically significant difference between patient and control groups, this polymorphism does not seem to play a role in the occurrence of recurrent pregnancy loss. Conclusion: As a result of the study we may assert that PAI-1 4G/5G and MTHFR A1298C and C677T polymorphisms are not risk factors for recurrent pregnancy losses in Mus city.

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Recurrent Pregnancy Loss , MTHFR , C677T , A1298C , PAI , 1 4G , 5G , Polymorphism

Kocatepe Medical Journal

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Kocatepe Medical Journal